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Figure 3


Fig. 3. Muscle patterning and differentiation defects in CA-ß-catenin/Wnt1-Cre mutant embryos. (A,A') Whole-mount in situ hybridization (ISH) for Dlx5 in control and CA-ß-catenin/Wnt1-Cre mutants, n=3. Arrowheads point to the CNC cells in the branchial arches (ba1 and ba2). (B,B') Capsulin ISH in control and mutants, n=6. Arrowheads point to the muscle anlagen in the branchial arches. (C,C') Tbx1 ISH, n=6. (D,D') Myf5 ISH, n=5. (E,E') MyoD ISH, n=4. (F,F') Myogenin ISH, n=4. Open arrowheads indicate on the loss of muscle anlagen. fl, forelimb; ht, heart; nt, neural tube. Scale bars: in A, 0.5 mm for A',D-D',E-E',F-F'; in B, 0.7 mm for B'-C'.