Fig. 4. Ablation of the CNC in chick embryos affects cranial muscle patterning. Images of control (A) or CNC-ablated (A') chick embryos at stage 8 (ablation boundaries are marked by white broken lines in A', dorsal view). (B-E') Whole-mount in situ hybridization in control and CNC-ablated embryos (lateral views) and the coronal/transverse sections (white broken line) of the corresponding embryos on the left: Tbx1 (B-B''', n=7/7), capsulin (C-C''', n=5/6), Myf5 (D-D''', n=8/9) and MyoD (E-E''', n=5/5). Arrowheads point to the muscle anlagen in the branchial arches (ba1 and ba2) and open arrowhead indicates their absence. ht, heart; nt, neural tube; ov, otic vesicle. Scale bars: in A, 0.4 mm for A'; in B, 0.5 mm for B'-E'.