Fig. 3. Specificity of the anti-LIO antibody. Wild-type (A) and lio²/drl^R343 (protein null) mutant (B) Drosophila third instar larval brains were double stained with anti-LIO (green) and anti-FASII (red). Panels A and B are reconstructions of three confocal sections of 1 µm. No crossreaction with another protein is detected in null individuals. Some background staining may occur in the null individuals, but its localization and aspect are clearly unrelated to the true labeling seen in the wild type. The wild-type left hemisphere is somehow twisted, and its MB is not in the same focal plan, but it appears normally in subsequent confocal sections. Scale bars: 100 µm.