Fig. 9. MB defects of Drosophila Wnt5 LOF mutants are rescued by Wnt5⁺ expression in the MBs. (A-C) Composite confocal images of MB phenotypes visualized with the GAL4-c739 line combined with UAS-mCD8-GFP. (A) In the Wnt5 null mutation, there is a complete arrest of axonal growth at the level of the peduncle, although Kenyon cells appear to be unaffected. The genotype is Wnt5⁴⁰⁰/Y; GAL4-c739 UAS-mCD8-GFP/+. B (excluding Kenyon cells) and C (including Kenyon cells) are images from the same brain and show a complete rescue of the MB mutant phenotype. The genotype is Wnt5⁴⁰⁰/Y; GAL4-c739 UAS-mCD8-GFP/+; UAS-Wnt5⁺/+.