Fig. 6. ERL1 and ER expression patterns. (A-H) In situ hybridization in wild-type Arabidopsis using an ERL1 (A-D) or ER (E-H) antisense probe. Both show broad expression in ovules with increased signal intensity in the chalazal region (A,E, arrowheads) and developing integuments (B,F, asterisks). Ovule is outlined in B. (D,H) Signal is still visible at maturity. (I-P) Comparison of ERL1 (I,J,M,N) or ER (K,L,O,P) mRNA accumulation in wild type and ER-family mutants examined by in situ hybridization. (Q) Real-time RT-PCR analysis of ER, ERL1 and ERL2 in stage 12 carpels of wild type and ER-family mutants. Transcript abundance of ER, ERL1 and ERL2 are higher in the absence of the other family members. Scale bars: 20 µm.