Fig. 1. S. mediterranea innexin gene expression analysis.
(A) Representative S. mediterranea innexin expression patterns
in wild-type animals were organized into four groups: (1) nervous: central
nervous system (CNS) and marginal cells (smedinx-3 and
smedinx-4), CNS and pharynx opening (smedinx-5); (2)
mesenchymal tissue (smedinx-1, smedinx-2,
smedinx-6, smedinx-7, smedinx-8,
smedinx-11 and smedinx-12), which is widely distributed and
might include more than one cell type (e.g. pharynx, CNS and diverse
sub-epithelial cells); (3) excretory system: flame cells
(smedinx-10); and (4) digestive: gastro-vascular system
(smedinx-9). In all panels, anterior is up and, except for the CNS
group, which is ventral, dorsal views are shown. For each probe,
n
18 animals (results were consistent in each case). (B)
Anterior transverse section (8 µm) at the level of the photoreceptors/brain
showing specific expression of smedinx-11 in individual cells. Red
arrowheads indicate representative expressing cells. CG, cephalic ganglia; PR,
photoreceptor. Scale bars: 0.2 mm in A; 20 µm in B.