Fig. 6. F-actin and N-cadherin distribution in dispase-isolated PSMs cultured with and without fibronectin. Somite III regions (i.e. somite -II at 0 hours) of dispase-isolated chick PSMs after 6 hours of culture in control medium show only small foci of F-actin (A) and N-cadherin (D) clustering. By contrast, the same region of dispase-isolated PSMs cultured with fibronectin shows a prominent apical enrichment of F-actin (B) and N-cadherin (E) and a nuclear alignment and elongation typical in epithelial somites (B,E). Interestingly, this cellular arrangement is very similar to that observed in collagenase-treated PSMs (C,F). Anterior, left. Scale bars: 25 µm.