Fig. 8. A 70 kDa peptide containing the fibronectin assembly domain inhibits
somitogenesis in embryo explants cultured for 12 hours. (A-D)
Ventral view of chick embryo explants cultured with BSA (control) or the 70
kDa fibronectin fragment. Endoderm was removed on the left while the other
side was kept intact. Embryos incubated with BSA form the expected number of
somites in 12 hours (A,C), whereas embryos cultured with the 70 kDa
fibronectin fragment form fewer somites (B,D). When embryos are cultured with
100 µg/ml of the peptide, somitogenesis is practically abolished (D).
(E) Bar chart of the results obtained in A-D. Error bars represent 95%
confidence limits. *, Somites formed in culture; BSA, bovine serum
albumin; 70 kDa, 70 kDa fibronectin fragment; ns, not significant;
**, P
0.005. Scale bars: 100 µm.