Fig. 6. ß-catenin associates with chromatin in promoters of TCF/Lef target genes in vivo. ChIP was performed on rat E15.5 kidneys. (A) Chromatin was cross-linked and DNA was fragmented to 400 bp. (B) Subsequently, ß-catenin-containing DNA-protein complexes were immunoprecipitated as verified by immunoblotting. (C) Following reversal of cross-links, a strong enrichment of promoter sequences containing TCF/Lef sites (targets, indicated in diagrams on the left) was observed in ß-catenin immunoprecipitates (ß-catenin IP) but not control IP (no antibody). Off-target control sites were not enriched, demonstrating specificity.