Fig. 6. Pds5B^-/- MEFs lack sister chromatid cohesion defects. (A,B) Metaphases from mouse embryonic fibroblasts (MEFs) derived from WT and Pds5B^-/- mice show no differences in sister chromatid cohesion. (C) Multi-species alignment of AT hook domains of PDS5B. Hs, Homo sapiens; Pa, Pan troglodytes; Ca, Canis familiaris; Mm, Mus musculus; Rn, Rattus norvegicus; Ga, Gallus gallus; Xe, Xenopus tropicalis; Da, Danio rerio; Te, Tetraodon nigroviridis. (D) Subcellular localization of human homologs of PDS5 using EGFP-tagged PDS5A and PDS5B fusion proteins. HeLa cells were transfected with EGFP-PDS5A and EGFP-PDS5B using Lipofectamine. At 24 hours later, the EGFP signals were examined. Left panels are phase-contrast images of HeLa cells; right panels are images of GFP signals. Note that EGFP-PDS5B is present in the nucleus and highly enriched in the nucleolus (arrows), whereas EGFP-PDS5A is localized to the nucleus but excluded from the nucleolus (arrows). Scale bar: 5 µm.