Fig. 6. Sorted mesencephalic ventral midline cells generate mesDA neurons, but caudal FP cells do not differentiate into neurons in vitro. (A) HuC/D⁺ neurons are generated from E13.5 rat mesencephalic Corin⁺ cells, but not from metencephalic Corin⁺ cells at 6 DIV. (B) Neurons generated in mesencephalic Corin⁺ cell cultures express Nurr1. (C,D)Th⁺ and Pitx3⁺ mesDA neurons are efficiently generated from mesencephalic Corin⁺ cells, but not from mesencephalic Corin-negative cells. (E) Co-culture of Corin⁺ cells and DiI-labeled Corin^- cells. At 6 DIV, DiI⁺ cells do not account for the majority of HuC/D⁺ and Th⁺ neurons, suggesting that mesDA neurons generated in Corin⁺ cell cultures are derived from Corin⁺ cells, but not from contaminated Corin^- cells.