Fig. 3. Sox10 expression levels and early development of neural crest derivatives in Sox10^K2/K2 and Sox10^aa1/aa1 embryos. (A) Comparison of Rpl8-normalized Sox10 expression levels in wild-type (set to 1, indicated by dashed line) and age-matched Sox10^aa1/aa1 and Sox10^K2/K2 mouse embryos at 10.5 and 11.5 dpc following quantitative RT-PCR with primer pairs 1,2 (white bars) and 3,4 (black bars). Experiments were repeated three times with material from two embryos for each genotype and age. (B) Immunoprecipitation of Sox10 from extracts of wild-type (wt) and age-matched Sox10^aa1/aa1 (aa1/aa1) embryos at 11.5 dpc. Immunoprecipitated material was detected by western blotting with anti-Sox10 rabbit antiserum. Sox10, Sox10-containing extract from transiently transfected Neuro2a cells. (C) Whole-mount in situ hybridization of wild-type (wt), Sox10^K2/K2 and Sox10^aa1/aa1 embryos at 10.5 dpc and 11.5 dpc using an antisense riboprobe directed against Sox10. V, trigeminal; VII/VIII, facial/acoustic; IX, glossopharyngeal; X, vagus; ot, otic vesicle.