Fig. 5. Melanocyte development in Sox10^K2/K2 and Sox10^aa1/aa1 embryos. (A) Whole-mount in situ hybridizations were performed on wild-type, Sox10^K2/K2 and Sox10^aa1/aa1 mouse embryos at 12.5 dpc using antisense riboprobes against Dct and c-Kit. (B,C) Dct-positive and c-Kit-positive cells were quantified in the hindlimb region of wild-type embryos (black bars) and their Sox10^K2/K2 (B) or Sox10^aa1/aa1 (C) littermates (white bars). Three embryos were counted for each genotype. The number of cells counted in the wild type was set to 100%. Cell numbers for the Sox10^K2/K2 and Sox10^aa1/aa1 embryos are presented relative to the wild type as mean±s.d. Differences were statistically significant between wild type and each mutant as well as between the two mutants as determined by Student's t-test (P0.001).