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Figure 4


Fig. 4. FoxF function is not required for Hand-like expression in the TVCs. Electroporated Ciona embryos hybridized with the Hand-like probe (green) and the B7.5 lineage cells visualized with an anti-ß-galactosidase antibody (red). (A-A'') Wild-type embryo electroporated with Mesp>lacZ alone. Hand-like is expressed in anterior trunk ventral cells (TVCs), but not in posterior tail muscles. (B-B'') Embryo co-injected with Mesp>lacZ and FoxF morpholino. TVC migration is inhibited, but Hand-like expression persists in the anterior cells. (C-C'') Embryo co-electroporated with Mesp>lacZ and Mesp>FoxF:WRPW. TVC migration is inhibited, but Hand-like exhibits normal expression in two anterior B7.5 cells. (D-D'') Embryo co-electroporated with Mesp>lacZ, Mesp>Ets:VP16 and Mesp>FoxF:WRPW. Hand-like expression is present in all B7.5 cells even though migration is inhibited by FoxF:WRPW.