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Figure 1


Fig. 1. Dominant-negative SNAP25{Delta}20 blocks exocytosis. Xenopus ocytes were injected with either wild-type or SNAP25{Delta}20 mRNA (5-10 ng) and membrane capacitance was measured [Cm(nF)] using two electrode-voltage clamp over time, as indicated (mean±s.e.; n=8-20). Western blot analysis using SNAP25 antibody shows similar levels of expression of wild-type and SNAP25{Delta}20, and no band was detected in uninjected oocytes. SNAP25{Delta}20 runs faster than wild-type full-length SNAP25 because of the deletion of the last 20 residues. Ooc, uninjected oocytes; WT, wild type.