Fig. 1. Antibodies generated against chick Wnt1 and Wnt3a can be utilized for western blots and immunohistochemistry. (A) Western blot analysis of 293T cells transfected with control, Wnt1 and Wnt3a shows that antibodies specifically immunoreact with their cognate Wnt protein. A cluster of Wnt proteins migrates at approximately 40 kDa. The Wnt3a blot was also probed with anti-tubulin antibodies as a loading control. (B) Immunohistochemical analysis of COS cells transfected with Wnt1 or Wnt3a. Wnt1/Wnt3a were visualized by the addition of Cy3-labeled secondary antibody (green); nuclei were visualized with DAPI (blue).