Fig. 2. kgb-1 mutants have more GLH-1 with grossly expanded P granules. (A) Schematic of the adult C. elegans germline adapted from Navarro et al. (see Navarro et al., 2001; Smith et al., 2002). Both gonad arms are shown, containing mitotic nuclei, the transition zone from mitosis to meiosis, the pachytene region and the diakinetic region where oocytes cellularize. DTC, distal tip cell; D, distal gonad; E, embryo; U, uterus; S, sperm; P, proximal gonad; O, mature oocyte. (B) One arm of a splayed gonad from an N2 worm grown at 26°C for 2d>L4 (DAPI-stained). Germ cell nuclei in pachytene (arrowhead) and diakinesis (asterisk) are indicated. (C) A kgb-1 mutant treated as in B shows one gonad arm wrapped on itself, with the distal end at the top and the proximal end to the right (DAPI). Examples of EMO (endomitocially-replicating) nuclei are indicated with arrows. (D) Gonad in B with anti-GLH-1 antibody. (E) Gonad in C with anti-GLH-1 antibody. (F) Magnified view (1000x) of the pachytene region of the N2 gonad (the boxed region from D). (G) Magnified view (1000x) of the kgb-1 gonad (the boxed region from E).