Fig. 3. KGB-1 is uniformly cytoplasmic in the distal gonad, aggregating into dispersed particles when oocytes cellularize. (A) A splayed gonad of a young N2 adult C. elegans (DAPI-stained). (B) The gonad in A reacted with anti-GLH-1 (red). (C) The gonad in A, with anti-KGB-1 (green). (D) A merged image of B and C. (E) Magnified view (1000x) of maturing oocytes (the boxed region from D). (F) A splayed gonad from a young kgb-1(km21) worm (DAPI). (G) The gonad in F with anti-GLH-1. (H) The gonad in F with anti-KGB-1. The kgb-1(km21) strain (Mizuno et al., 2004) was used in F-H to establish the specificity of the N-terminal KGB-1 antibody as this region is deleted in kgb-1(km21), but not in kgb-1(um3).