Fig. 1. Defects in mesoderm spreading in how germline clone embryos. Whole-mount wild-type (WT; A-E) or how germline clone (F-J) embryos stained for Twist (TWI; A,F), Tinman (TIN; B,G), EVE (C,H), EVE (green) + MEF2 (red) (D,I) (D' and I' represent respective single EVE staining of the merged images in D,I), and Myosin heavy chain (MHC; E,J). Arrows indicate uneven mesoderm spreading (F), segments lacking TIN-positive heart cells (G), segments lacking EVE-positive cells (H), uneven distribution of cardioblasts (I) and segments lacking dorsal muscles (H). Arrowhead in I shows a region lacking both cardioblasts and EVE-positive pericardial cells, while more ventral MEF2-positive cells are present. The images in D-I' were taken in threefold higher magnification to detect the details of the distinct cell types.