Fig. 3. The four mRNA targets of HOW are elevated in the mesoderm of how germline clone embryos and exhibit direct binding to HOW. Whole-mount wild-type (A-D) or how germline clone (E-H) embryos hybridized with each of the four mRNAs identified in the microarray screen: miple (A,E), lap (B,F), CG31638 (C,G) and falten (D,H). Arrowheads in E-H indicate the specific increase in the levels of the distinct mRNAs in the mesoderm of how germline clone embryos. (I) HOW-RNA binding assay with in vitro-transcribed RNA of the 3' UTRs of miple, lap, CG31638, falten and punt. HOW^R185C is a form of HOW that is mutated in the KH RNA-binding domain and served as a control for non-specific binding. The first two lanes on the left represent total HOW protein levels. Each binding experiment shown is representative of three repetitions.