Fig. 2. Knockdown of either RGA-3 or RGA-4 leads to a hyper-contractile cortex. (A) Schematic drawing of the RNAi constructs used: for rga-3, apart from the initially cloned N-terminal sequence, constructs matching the middle part and to the 3'UTR of the gene are chosen; for rga-4, an RNAi targeting the middle part of the gene is also created. Exons are indicated in white stretches; introns in yellow. Introns are bigger in the Y75B7AL.4 gene (rga-4) compared to K09H11.3 (rga-3). (B) Pictures of Nomarski time-lapse studies after feeding with bacteria expressing dsRNA of the different RNAi constructs. For all constructs, a membrane-ruffling phenotype was observed. s, seconds. Scale bar: 20 µm.