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Figure 5


Fig. 5. Hyper-contractility in rga-3/4(RNAi) embryos is dependent on NMY-2, and NMY-2::GFP is enriched at cortical membrane ruffles in rga-3/4(RNAi) embryos. (A) nmy-2(RNAi) rescues the membrane-ruffling defect of rga-3/4(RNAi). All three proteins were knocked down at the same time by feeding. (B) Time-lapse images of NMY-2::GFP behaviour in wild-type and rga-3/4(RNAi) embryos. NMY-2::GFP is strongly enriched in the pseudo-cleavage furrow and at the anterior cortex after rga-3/4(RNAi). (C) Quantification of the fluorescence intensity of NMY-2::GFP using ImageJ. (D) Quantification of the length of the NMY-2::GFP domains at the plasma membrane in wild-type (n=7) and rga-3/4(RNAi) (n=9) zygotes. Scale bar: 20 µm.