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Figure 6


Fig. 6. Polarity establishment is not affected in rga-3/4(RNAi) embryos but the anterior PAR-6-domain size fluctuates more in rga-3/4(RNAi) embryos compared with wild type. (A) GFP::PAR-2 and (B) GFP::PAR-6 localization is determined in vivo in wild-type and in rga-3/4(RNAi) embryos. GFP::PAR-2 as well as GFP::PAR-6 is correctly localized in rga-3/4(RNAi) embryos at the posterior and anterior cortex, respectively. (C) The relative size of the GFP::PAR-2 (wild type: n=24 embryos; RNAi: n=18) and GFP::PAR-6 (wild type: n=22; RNAi: n=25) domains was determined by drawing a line from the anterior to the posterior end of the embryo and then measuring the size of the individual domain. The size of the GFP::PAR-6 domain fluctuated more in rga-3/4(RNAi) embryos, compared with wild type. The variations in the domain sizes are statistically significant (P<0.01). The size of the GFP::PAR-2 domain was less affected. Scale bars: 20 µm.