Fig. 8. rga-3/4(RNAi) causes strong gonad defects in let-502(sb106) mutants. (A) Gonad arms were extracted from worms and stained with rhodamin-phalloidin and DAPI. The gonads are oriented with the distal tip pointing towards the left. A schematic drawing of the gonad is included. The arrowheads point to the rachis formation defects. The arrow points to the `hole' in the let-502(sb106) rga-3/4(RNAi) gonad. (B) A gonad from the let-502(ok1283) mutant, which represents a knock-out allele, was stained as in A. The arrow points to the `hole'. (C) Quantification of gonad defects of N2 and let-502(sb106) animals treated with rga-3/4 or cyk-4 dsRNA. The number of gonads used for quantification of a particular phenotype is given in parenthesis. Depending on how the gonads adhered to the glass surface, not all phenotypes could be quantified in the same gonad. Ambiguous-looking gonads were not included in the analysis. Scale bar: 50 µm.