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Figure 2


Fig. 2. Proteasomal `speckles' associated with the individualization complex. (A) Elongated spermatid bundles showing Pros{alpha}6T-GFP speckles (arrows, green). (B-E) Speckles (green) are located close to the actin cones (red): (B) Pros{alpha}6T-GFP, (C) Pros{alpha}6-GFP, (D) Pros{alpha}3T-GFP, (E) Pros{alpha}3-GFP. (F) Wild-type IC stained with anti-Pros{alpha}7 antibody (green) and Alexa Fluor 635 phalloidin (red). (G-I) Pros{alpha}6T-GFP (G, green) in an IC stained with anti-Pros{alpha}7 antibody (H, red). (I) The merged image of G and H with the yellow signal indicating colocalization. Scale bars: 20 µm.