Fig. 3. Generation of the Pros
6T1
mutant. (A) Pros
6T knockout construct. The
bent arrow to the left of the Pros
6T coding region
(hatched area) indicates the transcription direction. The two arrows beneath
the Pros
6T coding region indicate the positions of
PCR primers used to verify the targeted Pros
6T
mutation. (B) Results of male fertility tests showing mean number of
progeny per male±s.e.m.