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Figure 5


Fig. 5. The individualization complexes are disrupted in Pros{alpha}6T1 mutant testes. (A-D)Testes stained with Alexa-Fluor-phalloidin (green) showing actin cones of the ICs. (A,B) Actin cones (green) in wild-type ICs move synchronously, although occasionally a few actin cones lag behind (arrow). (C,D) In Pros{alpha}6T1 testes, actin cone synchronization is disrupted. The further the ICs move along the tail bundles, the more scattered the actin cones become (compare D with C). (E,F) Wild-type (E) and Pros{alpha}6T1 (F) testes stained with anti-Pros{alpha}7 antibody (green) and Alexa-Fluor-phalloidin (red). Speckles (arrow) can be observed in the wild-type testis, whereas they are absent in the Pros{alpha}6T1 testis. Two cystic bulges are shown in F. The actin cones are disorganized as a result of the mutation. Scale bars: 20 µm.