Fig. 6. Caspase activation is affected in Pros6T¹. (A,B) Anti-active Dronc staining (red) is observed in cystic bulges in wild-type testes (A), whereas it is absent in Pros6T¹ testes (B). (C,D) Visualization of active Ice with anti-cleaved-caspase-3 antibody (CM1; red). CM1 staining is observed in the cystic bulge (arrow) and elongated spermatid bundles (arrowheads) in wild-type testes (C). However, CM1 staining is not detected in the cystic bulge (arrow) in Pros6T¹ testes (D), and is weaker in elongated spermatid bundles (arrowheads) compared with wild type. Actin cones were visualized by Alexa-Fluor-phalloidin staining (green). Scale bars: 20 µm.