Fig. 2. Expression of various gene markers of the A-line neural cells following inhibition of Nodal signals. Embryo treatment is indicated above the panels and the marker analysed to the left of the panels. For control neural plate-stage embryos, nuclei are labelled with Hoechst (white) to allow the easy identification of individual cells. For this and subsequent figures, black dots on the schematics mark blastomeres expressing each given gene and, unless otherwise stated in the text, the vast majority of embryos exhibited bilateral staining. Expression of Ci-MRF and Ci-Tbx6b in A9.31 is indicated by an arrowhead on the right-hand side. For lateral markers, numbers indicate any expression over the total number of embryos analysed. For the medial marker cicl007j15, numbers indicate the mean number of cells (c) per embryo expressing a given gene. n, total number of embryos analysed.