Fig. 8. Expression of Ci-COE and Ci-MRF following UO126 treatment at different time points. Graphs show the percentage of embryos showing expression in A9.31 (row I/column 4). The key for the graph and scoring is the same as in Fig. 5. A sample of embryos was taken at the same time point that embryos were placed in inhibitor and analysed for Ci-Ngn expression to determine the percentage of embryos in each sample in which A8.16 had cleaved. These percentages are indicated on the graphs (arrows). On the x-axis: C, control; 110, 110-cell stage; eG, early gastrula stage; eG+30, 30 minutes after eG; eG+45, 45 minutes after eG. The minimum number of embryos analysed for each time point was 12 embryos for Ci-COE and 17 for Ci-MRF. This experiment was repeated two more times with similar results (data not shown).