Fig. 6. Overexpression of cPouV in cESC and mESC. (A) Following transfection of the different PouV coding sequences, overexpression of cPouV in cESC revealed loss of endogenous cNanog expression and an increase in Gata4, Gata6 and Cdx2 expression. (B) A similar profile was observed for XlPou91, but only a slight increase in Cdx2 when mOct4 is transfected in cESC. (C,D) Upregulation of Gata4, Gata6 and Cdx2 is strong (C) when XlPou91 is overexpressed in mESC with an induction of other markers (Hnf1, brachyury, Sox17 and laminin B1), in contrast to a moderate induction when cPouV is overexpressed in mESC (D). A value of 1 was given to the gene expression level obtained in clones transfected with the empty vector (empty). Two independent experiments provided similar results.