Fig. 2. Osk^imm dominantly influences polar granule and nuclear body morphology. Pole cells displaying GFP-Aub (green) and immunostained for Osk (red) from cellular blastoderm stage (A,D) or gastrulation stage (B,C,E,F) embryos, with enlarged views of nuclear bodies in C and F. Embryos are from otherwise wild-type mothers bearing a single copy of the following transgenes: A-C, P[osk⁺]; D-F, P[osk^imm]. P[osk⁺] polar granules are spherical (A,B). In the presence of both endogenous Osk and Osk^imm (D,E), the polar granules fuse into one to three large aggregates. Nuclear bodies are normally spherical (C), but in P[osk^imm] embryos (F) the nuclear bodies show deformations and discontinuities (arrows). Scale bars: A,B,D,E, 5 µm; C,F, 2 µm.