Fig. 6. NAB-1 is a presynaptic protein and partially co-localizes with SAD-1. (A) hpIs66 animals were co-stained with anti-GFP antibody (green) and either anti-SNT-1 (red, left panels) or anti-UNC-10 (red, right panels). (B) Wild-type animals co-stained with anti-SAD-1 antibody (red) and either anti-SNT-1 (green, left panels) or anti-UNC-10 (green, right panels). (C) hpIs66 animals were co-stained with anti-GFP (green) and anti-SAD-1 antibodies (red). (D) Young adult animals co-expressing SNB-1::mRFP (red) and NAB-1::GFP (green, left panels) or UNC-10::mRFP (red) and NAB-1::GFP (green, right panels) in GABAergic neurons. (E) Young adult animals co-expressing SAD-1::mRFP (red) and SNB-1::GFP (green) (left panels) or SAD-1::mRFP (red) and UNC-10::GFP (green) (right panels) in GABAergic neurons. (F) Wild-type animals co-expressing SAD-1::mRFP (red) and NAB-1::GFP (green) in GABAergic neurons. Last panels of each column show the enlarged image of areas indicated by white boxes. Scale bar: 5 µm.