Fig. 4. Transcriptome analysis in K14-Gata3^-/- HF. (A-F) Schematic of HF (A) and Gata3 expression in innermost layers of IRS at P11 (B). Fresh-frozen skin sections of P11 wt (C,D) and K14-Gata3^-/- (E,F) before (C,E) and after (D,F) collecting HF tissue by laser-capture. RNA from the captured tissue was used for microarray analysis. DP, dermal papilla; He, Henle's layer of IRS; HS, hair shaft; Hu+Ci, Huxley's layer and cuticle of IRS; Me, medulla; ORS, outer root sheath. (G,H) Full mouse genome transcriptome analysis of laser-captured, K14-Gata3^-/- and wt HFs. (G) Pie chart of significantly over-represented biological processes in K14-Gata3^-/- as compared with wt HFs. (H) Significant gene expression changes in the K14-Gata3^-/- as compared with wt mouse HFs (for an extensive overview, see Table S1 in the supplementary material). FC, fold change; P=P-value for two-tail analysis of variance.