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Figure 2


Fig. 2. {alpha}-spectrin weakly contributes to midline axon guidance. (A-E) Late stage 16 embryos stained with anti-Fas2 Mab. Anterior is up. (A) Heteroallelic {alpha}-spectrin (lm88/rg41) mutant embryos on average contain less than one Fas2-positive ectopic crossover (arrow). (B) {alpha}-spectrin (Im88) heterozygous embryos do not exhibit midline guidance errors. (C) Similar to the heteroallelic combination, {alpha}-spectrin (Im88) homozygous embryos display mild midline guidance defects. (D) Removing one copy of {alpha}-spectrin (Im88) mildly enhances the ß-spectrin (G0198) hypomorphic phenotype. (E) Stronger genetic interactions are observed when two copies of {alpha}-spectrin are removed in ß-spectrin hypomorphic mutants. Note the increase in Fas2-positive longitudinal axon bundles crossing the midline. (F) Quantification of single and double mutants. a, {alpha}-spectrin(rg41)/ {alpha}-spectrin(lm88) (n=19); b, lm88/TM3ß (n=7); c, lm88/lm88 (n=11); d, ß-spectrin(G0198)/Y (n=14); e, G0198/Y; lm88/TM3ß (n=6); f, G0198/Y; lm88/lm88 (n=8). Asterisk denotes a significant difference between genotypes f and c, and f and d (P=2.86x10-5 and 0.000154 respectively; two-sample Student's t-test). Error bars indicate s.e.m.