Fig. 6. The level and localization of Robo are not altered in ß-spectrin null embryos. (A,D) Embryos stained with anti-HRP. (B,E) Embryos stained with anti-Robo. (C,F) Merged image for each genotype. ß-spectrin mutant embryos (D-F) do not show reduction or mislocalization of Robo, although the distance between opposing sides of the midline is reduced when compared with wild-type embryos (A-C). Embryos were processed in parallel and images were taken at the same confocal settings.