Fig. 8. Edn1-deficient ectoderm recapitulates the suc/edn1^-/-mutant phenotype. Whole-mount RNA in situ hybridizations (A,B) with immunohistochemistry for biotin-dextran (C,D). (A-C) Dorsolateral views. hand2 expression at 30 hpf (A) is lost in suc;edn1^-/- mutants (B). (C) Wild-type embryos that receive ectoderm from suc;edn1^-/- donors lose hand2 expression adjacent to the transplanted ectoderm (brown cells, arrow). (D) Transverse cryosection through the arches of the embryo in panel C show that donor cells are confined to ectoderm (arrow, brown cells).