Fig. 8. stripeA mRNA levels are specifically reduced in crn mutant embryos. (A) RT-PCR with stripeA- and stripeB-specific primers, performed on RNA extracted from crn homozygous mutant or from wild-type embryos at 14-16 hours AEL. The mRNA levels were normalized against tubulin mRNA levels in each sample. A specific reduction of stripeA is detected. (B-E) Stage 16 wild-type (B) or crn mutant (D) embryos stained for Shot (red) and Stripe (Blue, C,D); C and D are the corresponding merged images. A significant reduction of Shot is detected in crn mutant embryos. (F-I) Stage 16 wild-type (F) or crn mutant (H) embryos stained for How (red) and Stripe (blue, G,I); G and I are the corresponding merged images. Note that there is no change in How levels in crn mutants. Arrowheads mark the tendon cells of the ventral longitudinal muscles.