Fig. 5. Lhx1;Lhx5 double-knockout mutants fail to maintain
inhibitory-neurotransmitter gene expression in the dorsal horn. (A-D)
Dorsal quadrants of the spinal cord at E12.5 and E13.5 showing the expression
of Viaat. In the DKO cord, a normal pattern of
Viaat expression in dILA neurons is observed up to E13.5.
(E,F) Beginning at E14.5, Viaat is downregulated in the
most-dorsolateral cells in the DKO cord (arrow in F).
(G,H) E17.5 DKO animals show a marked loss of
Gad1 expression in the dorsal regions (arrow in H).
(I,J) VGluT2 expression is not altered in the
DKO dorsal horn at E17.5.