Fig. 8. Pax2 maintains Lhx1 and Lhx5 expression in dIL_A interneurons. (A-D) Expression of Lhx1 and Lhx5 in the Pax2^-/- cord. Lhx1 and Lhx5 antibody stainings showing Lhx1 and Lhx5 expression in the dorsal neurons is Pax2-independent at E11.5 (A,B) and E12.5 (C,D). (E-H) In situ hybridization analysis of Lhx1 (E,F) and Lhx5 (G,H) at E14.5 shows that both genes depend on Pax2 for maintenance in the dIL_A population. (I-N) Viaat expression is correctly initiated in the Pax2^-/--mutant cord (I,J), but begins to be downregulated dorsally at E12.5. The dIL_A population shows a downregulation of Viaat at E12.5 (arrow in L). Viaat expression in the dorsal horn is largely missing by E14.5 (arrow in N) (see also Cheng et al., 2004). Notice that Viaat is downregulated dorsally, but remains largely intact in the ventral cord.