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Figure 2


Fig. 2. Verification of known Hox downstream genes identified by microarray analysis. (A) Relative expression levels of seven known Hox downstream genes identified in a microarray screen are shown. For three of the seven genes, in situ hybridizations were performed. Genes shown are: reaper (rpr), paired (prd), ß-Tubulin at 60D (ß-Tub60D), Distal-less (Dll), spalt major (salm), empty spiracles (ems) and nubbin (nub). (B) {alpha}-Dfd and {alpha}-Abd-B antibody stainings on embryos misexpressing different Hox genes confirmed posterior suppression as seen in the microarray experiment. Antibody stainings for all (Abd-B misexpression) or for some (Dfd misexpression) Hox proteins are shown.