Fig. 3. Verification of newly identified Hox downstream genes by in situ hybridization. In situ hybridizations of the indicated genes in stage 11 and/or stage 12 arm::lacZ, arm::Hox, wild-type and Hox mutant embryos. Genes shown are: skl (A-D), CG5080 (E-H), CG7447 (I-L), sage (M-P), ImpL2 (Q-T) and spz (U-X). Hybridizations on embryos misexpressing Hox genes and on Hox mutant embryos were performed independently (with the respective arm::lacZ and wild-type controls). Differences in staining intensities are due to differences in the in situ hybridization procedures. Pictures of arm::lacZ and wild-type embryos were taken at different focal planes and thus expression patterns in these embryos appear slightly different. Red arrows mark expression domains changed in wild-type and Hox mutant embryos.