Fig. 5. Specificity of Hox downstream gene regulation. (A) Classification of Hox downstream genes according to their regulation by one (unique), several (regional) and all (common) Hox proteins. Numbers of unique downstream genes for each Hox protein are shown on the left. The distribution of classes does not change when the Abd-A experiment, which was performed independently, is excluded from the analysis (shown in the table). (B) Distribution of the regulatory classes among all identified Dfd downstream genes and predicted direct Dfd target genes is very similar. No commonly regulated downstream genes are found among the predicted direct Dfd target genes. (C) Distribution of Hox downstream genes regulated at the two different stages analyzed. On the left side, the percentage of all Hox downstream genes regulated at the two stages are shown; on the right side, the distribution for each individual Hox protein is indicated. Numbers of genes are shown within bars. (D) In situ hybridizations of selected examples of genes regulated at specific stages (early-specifc, early and late, late-specific). Expression patterns of the following genes are displayed: Heat shock protein 23 (Hsp23), CG3097 and wrapper.