Fig. 7. Augmented endothelial generation by GATA2. (A) GATA2 induction scheme in differentiating EBs and formation of endothelial cells. Uppermost numbers indicate EB day. Gray bar indicates duration of Dox treatment and subsequent analyses of D6 EBs. (B) Generation of Tie2⁺ endothelial cells by GATA2 expression in serum and SR conditions. Numbers in boxes indicate percentage of Tie2⁺ cells. (C) Generation of CD31⁺ and CD31⁺VE-Cadherin⁺ endothelial cells in SR media. Numbers in upper left and right quadrants indicate percentage of CD31⁺ and CD31⁺VE-Cadherin⁺ cells, respectively. (C') Quantification of CD31⁺ and CD31⁺VE-Cadherin⁺ cells generated with or without Dox. Values are mean±s.e.m. from four independent experiments; ^*, P<0.001 versus untreated. (D-E') Representative sprouting EB images. D and E show 100x magnification of brightfield images of EBs generated in the absence (D) and presence (E) of Dox; D' and E' are 800x magnifications of D and E, respectively, showing the tendril-like (D') and endothelial (E') cells generated in the two conditions. (F,F') Brightfield (F) and fluorescence (F') images of 800x magnification of sprouting iGATA2 EBs demonstrating uptake of DiL-Ac-LDL by endothelial cells. (G) Fractional quantification of types of sprouting structures grown by 44 (-Dox) and 46 (+Dox) EBs grown in sprouting media.