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Figure 2


Fig. 2. Skeletal defects of E19 {Delta}IG-DMR/+ embryos. Comparison of skeletal defects between E19 maternally transmitted IG-DMR knockout embryos (B,D; KO/+) and wild-type littermates (A,C; +/+). Preparations are stained with Alcian Blue for cartilage and Alizarin Red for bone. An upward slanting thoracic cage, with wider angulation of the ribs relative to the sternum, is observed in B as compared with A. Eight ribs (instead of 7) were attached to the sternum for IG-DMR knockout embryos (compare D with C). The green arrow indicates the extraossification observed in KO/+ embryos at the site where the 6th to 8th ribs attach to the sternum (D). The numbers in C and D refer to the costal cartilages, where the anterior-most rib is defined as 1. c, costal cartilage; ste, ossification centre of one of the sternebrae; xp, xiphoid process. Scale bar: 1 mm.