Fig. 1. Fgf3 alleles and adult phenotypes. (A) Depiction of
wild-type (Fgf3+), original (Fgf3neo)
and newly targeted (Fgf3
2)
Fgf3 alleles. Solid lines, mouse genomic Fgf3 DNA; open
boxes, untranslated regions; solid boxes, protein-coding regions; gray box,
MC1Neo cassette. (B) Genotypes at weaning of
Fgf3+/neo and
Fgf3+/2
intercrosses. Numbers and percentage of total (in parentheses) for wild-type
(+/+), heterozygous (+/neo or +/2) and homozygous (neo/neo or
2/2; photographs to the right) offspring are shown. (C)
Auditory brainstem response (ABR) thresholds of
Fgf3+/2 intercross
offspring. Average thresholds (in decibels sound pressure level) for left (L,
blue) and right (R, red) ears of +/+ (n=7) and +/2
(n=12) animals are shown as boxes; bars indicate one standard
deviation. Homozygous mutant (2/2) ABR measurements are
presented as connected circles (left, blue; right, red) for individual
animals, with numbers in parentheses indicating the total number of mutant
animals with the specified phenotype. Low (D,E) and high
(D',E') magnification views of Hematoxylin and
Eosin-stained mid-modiolar sections of
Fgf3+/2 (hearing,
D,D') and
Fgf32/2
(deaf, E,E') ears. oC, organ of Corti; sv, stria vascularis; tm,
tectorial membrane. Scale bars: 50 µm.