Fig. 1. The loss of lin-35, dpl-1, efl-1 or efl-2 function results in a reduced level of constitutive germ cell apoptosis. (A) Time-course analysis of constitutive germ cell apoptosis, which was analyzed in C. elegans hermaphrodites of the indicated genotypes. Average numbers of apoptotic germ cells per gonad arm of four independent experiments are shown. Error bars represent standard deviations. For each experiment, a minimum of ten animals per genotype and time point was scored double blind. All strains analyzed were homozygous for the mutation ced-6(n2095). Except for the strain efl-1(n3318), all strains were also homozygous for the P_lim-7ced-1::gfp integration bcIs39. m⁺z^- indicates that animals analyzed were homozygous mutant progeny of heterozygous animals. The complete genotypes of dpl-1(n3316) and efl-1(n3318) animals were dpl-1(n3316) unc-4(e120); ced-6(n2095); bcIs39 and ced-6(n2095); efl-1(n3318), respectively. (B) Analysis of constitutive germ cell apoptosis 40 hours post the L4 stage. Average numbers of apoptotic germ cells per gonad arm of two independent experiments are shown. Error bars represent standard deviations. For each experiment, a minimum of ten animals per genotype was scored blind. All strains were homozygous for ced-6(n2095) and bcIs39. Mutations of lin-35, dpl-1 and efl-2 used were: lin-35(n745), dpl-1(n3643) and efl-2(tm2359).