Fig. 3. Analysis of ced-9, ced-4, and ced-3 expression in lin-35 and dpl-1 mutant gonads. (A) Quantitative real-time RT-PCR experiments using cDNAs isolated from gonads dissected from wild-type (+/+), lin-35(n745) or dpl-1(n3643) C. elegans. Average relative abundances of ced-9, ced-4 and ced-3 mRNAs of three independent experiments, each performed in triplicate, are shown. Error bars represent standard deviations. (B) Western analyses using proteins extracted from gonads dissected from wild-type (+/+), lin-35(n745) and dpl-1(n3643) hermaphrodites. The protein ß-actin [lin-35(n745)] or ß-tubulin [dpl-1(n3643)] was used as loading control. One representative experiment is shown for each. (C) Quantification of the abundance of CED-9 and CED-4 proteins in the gonad of wild-type (+/+), lin-35(n745) and dpl-1(n3643) animals. Quantification was performed using NIH image software. Average relative abundances of CED-9 and CED-4 protein of three [lin-35(n745) and two dpl-1(n3643)] independent experiments are shown. Error bars represent standard deviation.