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Figure 2


Fig. 2. {Delta}N89ß-catenin expression induces alveolar development. Immunohistochemistry demonstrating expression of casein in alveoli from endogenous Rag1-/- (A) and transplanted PR-/-;{Delta}N89ß-catenin (B) glands at 14.5 days of pregnancy. Arrows point to luminal secretions containing casein. Immunohistochemical analysis demonstrating high levels of NKCC1 expression in young wild-type virgin (C) and transplanted PR-/- (D) glands at 14.5 days of pregnancy. Low levels of NKCC1 expression are observed in alveoli (arrowheads) generated in PR-/-;{Delta}N89ß-catenin (E) and endogenous Rag1-/- (F) glands at p14.5. Insets show higher magnifications of the boxed areas and demonstrate NKCC1 expression along the basolateral cell borders of ductal cells (insets in C,D) and absence of NKCC1 staining in alveoli (insets in E,F). (G) An example of the criteria used to identify primary ducts, secondary branches and side-branches. (H) Table of the number of secondary and side-branches observed per 1000 pixels of ductal length in PR+/- (n=7 fields), PR+/-;{Delta}N89ß-catenin (n=4 fields), PR-/- (n=7 fields) and PR-/-;{Delta}N89ß-catenin glands (n=6 fields). Differences in side-branches are statistically significant between PR+/- and PR-/- glands (P=0.008) and PR+/-;{Delta}N89ß-catenin and PR-/-;{Delta}N89ß-catenin glands (P=0.003), but not between PR-/- and PR-/-;{Delta}N89ß-catenin glands (P=0.057). Scale bars: 50 µm.