Fig. 4. Absence of PR restricts
N89ß-catenin-induced
precocious development to ductal tips. Carmine-stained wholemounts show
precocious development along the lateral borders and tips of ducts in
PR+/-;
N89ß-catenin glands (A) and
terminally restricted precocious development in
PR-/-;
N89ß-catenin glands (B). Indirect
immunofluorescence for the N-terminal Myc tag epitope detected by the 9E10
antibody shows that transgene expression in all luminal cells is unaffected by
PR genotype (C,D). Scale bars: 0.3 mm in A,B; 100 µm
in C,D.